Human Alcohol Dehydrogenase 1 (ADH1) ELISA Kit

Cat.#:

JL15451

Regulatory Status:

RUO

Alternative:

ADH1A; Alcohol Dehydrogenase 1A(Class I)Alpha Polypeptide; Alcohol dehydrogenase subunit alpha

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

1.56-100ng/mL

Sensitivity:

0.72ng/mL

Specificity:

This kit recognizes Human ADH1 in samples. No significant cross-reactivity or interference between Human ADH1 and analogues was observed.

Sample Type:

Serum;plasma;tissue homogenates and other biological fluids

Assay Procedure:

Research Area:

Metabolism

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| ADH1

Alcohol dehydrogenase 1(ADH1) is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. The gene is monomorphic and predominated in the fetal liver, while the genes encoding the beta and gamma subunits are polymorphic and strongly expressed in the adult liver.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human ADH1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	85-101	96
Plasma(n=8)	91-105	102
Tissue Homogenates(n=8)	96-108	105

| Linearity

Samples were spiked with high concentrations of Human ADH1 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Tissue Homogenates(n=4)
1 : 2	Range (%)	84-95	87-96	90-110
1 : 2	Average (%)	91	93	96
1 : 4	Range (%)	89-103	87-108	105-115
1 : 4	Average (%)	94	98	108